Biochem/physiol Actions

Protein Target: Kappa / OP2 / KOP / KOR

Target Sub-Family: Opioid

GPCR Class: A

Cell Line Description

GPCR Cell Lines

Host cells: Chem-1

Components

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.

General description

EMD Millipore's Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. EMD Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Opiates derived from the opium poppy, Papaver somniferum, have been used for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, µ, κ, and d, that activate Gi/o to reduce intracellular cAMP levels. Most clinically used opioids function by activation of the µ opioid receptor. Activation of the κ opioid receptor by selective agonists also produces analgesia, primarily mediated by spinal sites, but causes dysphoria and psychosis instead of euphoria. The κ receptor at central and peripheral sites is also largely responsible for the anti-diarrheal effects of opiates (Dhawan et al., 1996). EMD Millipore's cloned human κ-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant κ expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway.

Other Notes

Limited to a single use and shall not be propagated and/or re-frozen by licensee.